restriction analysis造句
例句與造句
- primer-introduced restriction analysis-pcr, pira-pcr
以引物引入限制性內(nèi)切酶分析聚合酶鏈反應(yīng) - the restriction analysis of 28s rdna pcr product showed that no restriction polymorphism were observed for alu i in all tested isolates of pleurotus
亞側(cè)耳在這兩個(gè)區(qū)域的長(zhǎng)度則與其它供試菌株均不相同。 - one hundred and seven isolates belonging to 12 chinese armillaria species and their relatives occurring in japan, europe and north america were subjected to a restriction analysis of intergenic spacer region ( igs-rflp ) using four restriction enzymes . the analysis revealed a total of 36 different rflp patterns
用pcr擴(kuò)增12個(gè)中國(guó)蜜環(huán)菌生物種及其相關(guān)的歐洲、北美和日本蜜環(huán)菌種的107個(gè)菌株的基因間隔區(qū)(igs)序列，用alui、hae、hinfi、tagi4種內(nèi)切酶進(jìn)行消化，結(jié)果產(chǎn)生了36個(gè)不同的rflp圖譜類型。 - 6 . the restriction analysis of its amplified product showed that no restriction site was observed for bamh i in all isolates, the other tested enzymes ( alu \, hae iii, hinf i, taq i, hha i, msp i ) could distinguish p . cilrinopileatus from the other pleurotus species
6.[ts擴(kuò)增產(chǎn)物的限制性酶切分析結(jié)果表明，召til)，hl對(duì)所有供試菌株均無酶切位點(diǎn)，而另外6利，供試內(nèi)切酶(alul、haeitl、llllal、11infl、九式斗，i、tcl叮i)都能將金頂側(cè)耳與其它側(cè)耳菌株區(qū)分開。 - recovered the agarose and identified by agarose gelelectrophoresis . the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e . coli ( dh5a ) . the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained
pcr產(chǎn)物經(jīng)回收后，經(jīng)瓊脂糖凝膠電泳鑒定后，與pcambia1303連接并轉(zhuǎn)化大腸桿菌dh5a，陽性重組子經(jīng)pcr和限制性內(nèi)切酶酶切圖譜分析，表明已獲得海藻糖-6-磷酸合成酶基因的植物表達(dá)載體。 - It's difficult to find restriction analysis in a sentence. 用restriction analysis造句挺難的
- in this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem-t easy vector . after transforming e . coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem-3abc . comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent . the pgem-3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii-digested expression vector ptriex-4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl-d-galactoside ( iptg ), bacteria were detected by sds-page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33.5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴(kuò)增產(chǎn)物連接到pgem-teasy載體中，轉(zhuǎn)化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質(zhì)粒經(jīng)酶切鑒定、pcr分析以及確證性測(cè)序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國(guó)外參考序列相比，同源性在99以上。將重組質(zhì)粒pgem-3abc和表達(dá)載體ptriex-4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達(dá)載體ptriex-4neo中，通過酶切鑒定、pcr擴(kuò)增以及序列分析，發(fā)現(xiàn)克隆到ptriex-4neo載體上的片段于3abc基因708bp處出現(xiàn)了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導(dǎo)表達(dá)，收集菌液進(jìn)行sds-page電泳、westernblotting分析，結(jié)果表明，3ab基因在大腸桿菌中成功表達(dá)，其表達(dá)產(chǎn)物為分子量33.5ku的融合蛋白，并能被口蹄疫病毒陽性血清識(shí)別。經(jīng)薄層掃描分析，表達(dá)量占總蛋白量的26以上。 - in this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem-t easy vector . after transforming e . coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem-3abc . comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent . the pgem-3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii-digested expression vector ptriex-4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl-d-galactoside ( iptg ), bacteria were detected by sds-page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33.5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴(kuò)增產(chǎn)物連接到pgem-teasy載體中，轉(zhuǎn)化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質(zhì)粒經(jīng)酶切鑒定、pcr分析以及確證性測(cè)序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國(guó)外參考序列相比，同源性在99以上。將重組質(zhì)粒pgem-3abc和表達(dá)載體ptriex-4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達(dá)載體ptriex-4neo中，通過酶切鑒定、pcr擴(kuò)增以及序列分析，發(fā)現(xiàn)克隆到ptriex-4neo載體上的片段于3abc基因708bp處出現(xiàn)了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導(dǎo)表達(dá)，收集菌液進(jìn)行sds-page電泳、westernblotting分析，結(jié)果表明，3ab基因在大腸桿菌中成功表達(dá)，其表達(dá)產(chǎn)物為分子量33.5ku的融合蛋白，并能被口蹄疫病毒陽性血清識(shí)別。經(jīng)薄層掃描分析，表達(dá)量占總蛋白量的26以上。 - detection of antigen-binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen-binding affinity of positive clones screened out in the former procedure; these positive phages were examined by restriction analysis ( ecor i and hind iii ); competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use
x陽性克到駛知烘扳原kbbjg）濁對(duì)陽性克隆進(jìn)行限制隴臥賜析（uwi和hedlll）鑒定三用競(jìng)爭(zhēng)elisatoljmg7重組噬菌體抗體性克隆對(duì)mg7單扶與其相應(yīng)抗原結(jié)合忙的喇率，從中j）ed出對(duì)mg7單抗與期?？乖Y(jié)合有抑余j效應(yīng)的克隆用于進(jìn)一涉研究。 - a dna fragment of 348-bp amplified from the b subunit gene was cut into two dna fragment of 216 and 132-bp by haelli . endonuclease restriction analysis of the plasmid content with psti showed that strainas with the same result of southern-blot with spesific probe had the different cleavage pattern . the isolated 285-bpand 348-bp dna was ligated with plasmid puc18 . the ligation mixture was used to transform e . coli jm109and the transformants were plated on lb agar containing antibiotics . plasmid dna containing cloned genes were used for direct sequencing
提示1999年的疫情由不同的病原菌引起。另外使用針對(duì)志賀毒素2及其變種的引物對(duì)進(jìn)行pcr檢測(cè)、細(xì)菌染色體pst和pcr產(chǎn)物的hae、ras酶切分析，以及pcr產(chǎn)物的序列分析，發(fā)現(xiàn)2000年從江蘇省徐州市患者和家畜家禽糞便標(biāo)本分離的大腸桿菌o157：h7菌株僅僅攜帶slt2vha基因。 - culture of mg7 hybridoma cells and detection of antigen-binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
mg_7重組噬菌體抗體庫(kù)的構(gòu)建及鑒定從培養(yǎng)的mg_7雜交瘤細(xì)胞中提取并分離mrna，反轉(zhuǎn)錄成cdna；利用pcr分別擴(kuò)增mg_7單抗的重鏈及輕鏈可變區(qū)基因，并通過?dna連接子將二者連接起來形成mg_7單鏈抗體基因；將mg_7單鏈抗體基因插入pcantab5e；將連接產(chǎn)物轉(zhuǎn)化感受態(tài)tg1大腸桿菌，制備細(xì)菌形式的mg_7重組噬菌體抗體庫(kù)；通過菌落計(jì)數(shù)和限制性酶切分析(ecor和hind)評(píng)估m(xù)g_7重組噬菌體抗體庫(kù)的容量和重組率。